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Pregnancy Midstream Tests

Testing for Pregnancy >> Pregnancy Midstream Tests

Please Bookmark Instructions for Future Reference

For a Printable Version of Pregnancy Test Instructions, please click here.

Get Pregnant - 5 Essential Tips
Human chorionic gonadotropin (hCG) is a glycoprotein hormone secreted by the developing placenta shortly after fertilization and implantation of the egg. The appearance of hCG soon after conception and its subsequent rise in concentration during early gestational growth make it an excellent marker for the early detection of pregnancy. First morning urine usually contains the highest concentration of hCG and is therefore the best sample when performing the urine test. However, randomly collected urine specimens may be used (though you should "hold" your urine for a few hours before collecting a sample.


1. Remove the test stick from its foil pouch by tearing at the notch and take the cap off the test stick.

2. To perform the test, turn the test stick so the absorbent tip is pointing in a downward direction and hold the test stick in your stream of urine so the urine makes contact with the absorbent area. Hold it for at least ten seconds so that adequate urine goes into the absorbent part of the tip. Do not allow urine to splash into the result window. To prevent urine from splashing the result window, during application, place your thumb over the interpretation window while holding the test in the urine stream.

3. Re-cap the pregnancy test and lay the test flat on a horizontal surface with the result window facing upwards.

4.Depending on the concentration of hCG, positive results may be observed in as little as 40 seconds. However, to confirm negative results, the complete reaction time of 5 minutes is required. It is important that the background is clear before the result is read. Do not read results after the specified reaction time.


IMPORTANT: After you have completed the pregnancy test, whether you are pregnant or not, a rose pink color band will appear in the control portion (C) of the window. This indicates that the Midstream test is working properly. As urine initially passes through the viewing window, the test band may momentarily be visible. This is not necessarily indicative of a positive result. Interpret results only after the complete test reaction time of 5 minutes.

Positive: After the urine has been in the pregnancy test stick for five minutes, you can conclude that you are pregnant if a rose-pink color band appears in the "test" portion (T) of the window (this second color band will appear in addition to the first control color band). See the diagram below.

Negative: You can conclude you are not pregnant if no rose-pink color band is seen in the large test window within 5 minutes. Note: Even if a faint rose-pink color band is visible in the test window at five minutes, the test is positive.

pregnancy results

Invalid: No visible band at all. The control band will not appear if an insufficient volume of specimen is added into the test kit. Proper procedures may not have been followed in performing the test. Repeat with a new test kit. Please consult above instructions and follow precisely. *Important Note from Regarding Invalid Test Results: The most common reason for an invalid test result with a midstream test is that urine has splashed into the interpretation window. This prevents liquid from properly “wicking” up the test and through the band of dye. Please note that invalid results can nearly always be avoided by placing your thumb over the interpretation window while holding the test in the urine stream.

To Optimize the Use of Testing Products, please visit our online Pregnancy and Ovulation Test Info

Notes On Pregnancy Midstream Tests / Test Limitations and Terms of Use
- The Urine hCG Pregnancy Test is for in vitro diagnostic use only (for external use only).
- As with all diagnostic tests, a final clinical diagnosis should not be based on the results of a single test, but should only be made by a physician.
- A negative result obtained from a urine specimen collected from a mother in very early pregnancy may be due to an extremely low concentration of hCG. In such cases, the test should be repeated on a fresh specimen obtained two days later.
- If a urine sample is too diluted, it may not contain a representative urinary hCG concentration. If a negative result is obtained with a low specific gravity specimen and pregnancy is still suspected, obtain a first morning urine specimen and retest.
- Test instructions must be followed precisely to insure accurate results. Please read instructions and follow exact test procedure. Please check with your doctor to verify diagnostic results – or if you receive unexpected or inconsistent test results.

I Missed My Period! Discover why... Click here to learn why hCG (the hormone detectable by our tests) causes you to miss your period.

Store the kit below 28°C; do not freeze. Optional liquid components require refrigeration (4°C). Refer to individual expiration dates of components for stability.

1. Do not use test after the expiration dates.
2. Dispose of used testing devices in a proper biohazard container.
3. If testing device has been stored in a refrigerator, allow them to warm to room temperature before performing the test.
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