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Pregnancy Midstream Tests Instruction

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For a Printable Version of Pregnancy Test Instructions, please click here.

Human chorionic gonadotropin (hCG) is a glycoprotein hormone secreted by the developing placenta shortly after fertilization and implantation of the egg. The appearance of hCG soon after conception and its subsequent rise in concentration during early gestational growth make it an excellent marker for the early detection of pregnancy. First morning urine usually contains the highest concentration of hCG and is therefore the best sample when performing the urine test. However, randomly collected urine specimens may be used (though you should “hold” your urine for a few hours before collecting a sample.


1. The BFP Pregnancy Test can be performed at any time of the day. However, the first morning urine is preferable.

2. Remove the test from the pouch by tearing from the notch. Remove the cap.

3. Hold the test by the thumb grip. Place the absorbent tip of the test in your urine stream until it is thoroughly wet (at least 10 seconds). If you prefer, you can urinate into a clean, dry cup or container and perform the test by dipping the absorbent tip of the test into the urine for at least 15 seconds.

4. Lay the stick down flat with the results window facing up. Wait 3 minutes. If you wish, you can replace the cap to cover the absorbent tip.


IMPORTANT: After you have completed the pregnancy test, whether you are pregnant or not, a rose pink color band will appear in the control portion (C) of the window. This indicates that the Midstream test is working properly. As urine initially passes through the viewing window, the test band may momentarily be visible. This is not necessarily indicative of a positive result. Interpret results only after the complete test reaction time of 3 minutes.

Positive: If 2 pink lines appear in the test control window, the test is positive and you can assume you are pregnant. Even if the lines are very light in color, you can still assume you are pregnant.

Negative: If only 1 pink line appears in the test control window, the test is negative and you can assume you are not pregnant.

Invalid: If a pink line does not appear in the test control window, the test is invalid. Invalid results may be an indication that the absorbent tip was not thoroughly saturated in urine. In this case, either urinate on the absorbent tip for additional time or re-dip the absorbent tip for 15 seconds and wait 3 minutes to read results. If there is still no pink line in the test control window, the test is invalid and testing should be repeated in a few days with a new BFP Pregnancy Test. Follow the instructions exactly, making sure the absorbent tip is thoroughly saturated with urine. Each test is designed for a single use and should not be reused.

To Optimize the Use of Testing Products, please visit our online Pregnancy and Ovulation Test Info

Notes On Pregnancy Midstream Tests / Test Limitations
and Terms of Use
The test works only if the instructions are followed precisely. Although the test is highly accurate in detecting pregnancy, a low incidence of false results can occur. Check with your doctor if you get unexpected or inconsistent test results. Certain health conditions can also cause a false or irregular test result. The following factors should not affect the test results: alcohol, analgesics (pain killers), antibiotics, and birth control pills. The test should not be used after the expiration date. Do not use the test if the protective foil pouch is opened or damaged. BFP Pregnancy Tests, when performed by consumers, showed an accuracy of greater than 99% when compared to lab results. Pregnant women should not drink alcohol; if you have already used alcohol, stop; if you’re thinking about getting pregnant, abstain from alcohol. Alcohol consumption during pregnancy has been known to cause birth defects.

I Missed My Period! Discover why… Click here to learn why hCG (the hormone detectable by our tests) causes you to miss your period.

Store the kit below 28°C; do not freeze. Optional liquid components require refrigeration (4°C). Refer to individual expiration dates of components for stability.

1. Do not use test after the expiration dates.
2. Dispose of used testing devices in a proper biohazard container.
3. If testing device has been stored in a refrigerator, allow them to warm to room temperature before performing the test.

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